Two synthetic biology's tools

Authors

  • Carla Giménez Instituto de Ciencias Básicas y Medicina Experimental, Instituto Universitario del Hospital Italiano
  • Lucía A. Curti Universidad Nacional del Noroeste de la provincia de Buenos Aires, UNNOBA
  • Federico Pereyra-Bonnet Instituto de Ciencias Básicas y Medicina Experimental, Instituto Universitario del Hospital Italiano

DOI:

https://doi.org/10.51987/Rev.Hosp.Ital.B.Aires.v36i3.669

Keywords:

synthetic biology, CRISPR, RNAs synthetic messengers, regenerative medicine, cell therapy

Abstract

Synthetic biology came to settle in and break the boundaries of the science. Many molecular techniques overcome the fiction to become reality. This article discusses two innovative techniques, as CRISPR, in particular the application of CRISPR-on which is able to activated particular human genes, and the synthetic RNAs messengers for isolation and purification specific cells. From a gaze focused on translational medicine, both tools offer great therapeutic potentia

Downloads

Download data is not yet available.

References

Malyshev DA, Dhami K, Lavergne T, et al. A semi-synthetic organism with an expanded genetic alphabet. Nature. 2014;509(7500):385-8. DOI: https://doi.org/10.1038/nature13314

Hutchison CA 3rd, Chuang RY, Noskov VN, et al. Design and synthesis of a minimal bacterial genome. Science. 2016;351(6280):aad6253. DOI: https://doi.org/10.1126/science.aad6253

Makarova KS, Wolf YI, Snir S, et al. Defense islands in bacterial and archaeal genomes and prediction of novel defense systems. J Bacteriol. 2011;193(21):6039-56. DOI: https://doi.org/10.1128/JB.05535-11

Jinek M, Chylinski K, Fonfara I, et al. A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. Science. 2012;337(6096):816-21. DOI: https://doi.org/10.1126/science.1225829

Mali P, Yang L, Esvelt KM, et al. RNA-guided human genome engineering via Cas9. Science. 2013;339(6121):823-6. DOI: https://doi.org/10.1126/science.1232033

Yang L, Güell M, Niu D, et al. Genome-wide inactivation of porcine endogenous retroviruses (PERVs). Science. 2015;350(6264):1101-4. DOI: https://doi.org/10.1126/science.aad1191

Kaminski R, Chen Y, Fischer T, et al. Corrigendum: Elimination of HIV-1 Genomes from Human T-lymphoid Cells by CRISPR/Cas9 Gene Editing. Sci Rep. 2016;6:28213. DOI: https://doi.org/10.1038/srep28213

Liang P, Xu Y, Zhang X, et al. CRISPR/Cas9-mediated gene editing in human tripronuclear zygotes. Protein Cell. 2015;6(5):363-72. DOI: https://doi.org/10.1007/s13238-015-0153-5

de Groote ML, Verschure PJ, Rots MG. Epigenetic Editing: targeted rewriting of epigenetic marks to modulate expression of selected target genes. Nucleic Acids Res. 2012;40(21):10596-613. DOI: https://doi.org/10.1093/nar/gks863

Zhao Y, Schapotschnikow P, Skajaa T, et al. Probing lipid coating dynamics of quantum dot core micelles via Förster resonance energy transfer. Small. 2014;10(6):1163-70. DOI: https://doi.org/10.1002/smll.201301962

Giménez CA, Ielpi M, Mutto A, et al. CRISPR-on system for the activation of the endogenous human INS gene. Gene Ther. 2016;23(6):543-7. DOI: https://doi.org/10.1038/gt.2016.28

Lee RC, Feinbaum RL, Ambros V. The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14. Cell. 1993;75(5):843-54. DOI: https://doi.org/10.1016/0092-8674(93)90529-Y

Ruvkun G. Molecular biology. Glimpses of a tiny RNA world. Science. 2001;294(5543):797-9. DOI: https://doi.org/10.1126/science.1066315

Kim DS, Gusti V, Pillai SG, et al. An artificial riboswitch for controlling pre-mRNA splicing. RNA. 2005;11(11):1667-77. DOI: https://doi.org/10.1261/rna.2162205

Pereyra-Bonnet F, Gimeno ML, Argumedo NR, et al. Skin fibroblasts from patients with type 1 diabetes (T1D) can be chemically transdifferentiated into insulin-expressing clusters: a transgene-free approach. PLoS One. 2014;9(6):e100369 DOI: https://doi.org/10.1371/journal.pone.0100369

Orqueda AJ, Giménez CA, Pereyra-Bonnet F. iPSCs: A Minireview from Bench to Bed, including Organoids and the CRISPR System. Stem Cells Int. 2016;2016:5934782. DOI: https://doi.org/10.1155/2016/5934782

Miki K, Endo K, Takahashi S, et al. Efficient Detection and Purification of Cell Populations Using Synthetic MicroRNA Switches. Cell Stem Cell. 2015;16(6):699-711. DOI: https://doi.org/10.1016/j.stem.2015.04.005

Downloads

Published

2016-09-30

Issue

Vol. 36 No. 3 (2016): Revista del Hospital Italiano de Buenos Aires

Section

Desde el ICBME

License

Copyright (c) 2016 Revista del Hospital Italiano de Buenos Aires

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

How to Cite

1.
Giménez C, Curti LA, Pereyra-Bonnet F. Two synthetic biology’s tools. Rev Hosp Ital B.Aires [Internet]. 2016 Sep. 30 [cited 2026 Aug. 21];36(3):124-8. Available from: https://ojs.hospitalitaliano.org.ar/index.php/revistahi/article/view/669

Most read articles by the same author(s)